Araf I. Jabbar; Ali I. Khaleel; Mohammed Z. Thani
Abstract
Micro-extraction techniques with UV-Vis measurement have been developed and validated for the analysis of Cefixime (CFX) in pure and pharmaceutical form. In this work, the DLLME and ...
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Micro-extraction techniques with UV-Vis measurement have been developed and validated for the analysis of Cefixime (CFX) in pure and pharmaceutical form. In this work, the DLLME and SIHLLME methods were employed for the extraction, enrichment and evaluation of CFX in pure form and pharmaceuticals using UV-Vis spectroscopy at 274 and 282 nm, respectively. The type of dispersive and extraction solvents and their volumes, pH, the amount of salt, and the centrifuging time were among the experimental variables that were accomplished. Under the perfect conditions, the procedures were linear in the range of 5.0-100.0 and 1.0-15.0 μg/mL, with a correlation coefficient of determination (R2) of 0.9963 and 0.9984 for DLLME and SIHLLME, respectively, LOD was 3.7 and 0.11 µg/mL. Recovery of the target analyte in pharmaceuticals was 96.5%-102.4%. The method can be easily applied to CFX concentrations above this value, but needs further improvements before it can be used in pharmacokinetic studies where more sensitive methods can be found. A simple, inexpensive, accurate, and precise spectrophotometric assay of CFX in pharmaceutical formulations was developed and validated following DLLME and SIHLLME for routine analysis.
